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Master Sciences, Technologies, Santé
MENTION BIOLOGIE INTÉGRATIVE ET PHYSIOLOGIE

Genomics of marine organisms (6 ECTS)

Supervisors

        Thierry Tonon (tonon @ sb-roscoff.fr)
        Arnaud Tanguy (tanguy @ sb-roscoff.fr)

Objectives

Through a series of lectures and laboratory projects, we will demonstrate how genomics can be applied to study biological processes such as development, a response to biotic or abiotic stresses, metabolism, and evolution in marine organisms. An overview of the current knowledge in marine genomics and an introduction to structural, functional and comparative genomics is included. Analytical approaches such as sequencing, microarray, quantitative RT-PCR, and heterologous expression will be introduced and illustrated using examples selected from among marine organisms, mainly algae and invertebrates. Students will get hands-on experience using these techniques in laboratory projects conducted during the course.

Content

 

  • Overview of marine genomics, new findings in marine biology where genomics have had an important role
  • Techniques in marine genomics (sequencing, transcriptomic methods (cDNA libraries, quantitative RT-PCR, microarrays)), and managing genomic data (including genome and EST annotation)
  • Functional genomics: methods (heterologous expression, RNAi, transformation, and mutagenesis) and approaches for marine organisms
  • Specificities and constraints of the marine environment relative to the terrestrial environment
  • Biology and genomics of different models in marine biology for invertebrates (oyster, sea urchin, mussels, annelids) and algae (the red alga Chondrus crispus, the green alga Ostreococcus tauri, and the brown alga Ectocarpus siliculosus)
  • Seminar series on different topics in genomics (structural genomics, functional genomics and comparative genomics)
  • Lectures will be completed by practical work, emphasizing techniques such as annotation of genes from marine eukaryotes and prokaryotes, manipulation of microarray data, use of specific software (phylogeny, polymorphism), RNA extraction, and quantitative RT-PCR and microarray experiments (pre and post-hybridisation)

Laure-Anne Nemirouvsky - 01/12/15

Traductions :

    Contact

    Master Biologie intégrative et physiologie

    9 quai Saint Bernard

    75252 Paris cedex 05

    Bât. B - 3e étage

    Porte 314 - BC 118

     

    Coordonnées de la direction et de l'équipe pédagogique